Isolasi Bakteri Selulolitik Pada Ayam Kampung (Gallus gallus domesticus) Sebagai Kandidat Probiotik Ternak Unggas
DOI:
https://doi.org/10.29303/jmbc.v1i3.8278Kata Kunci:
Cellulolytic Bacteria, Local Chicken, Probiotics, Gallus gallus domesticus, Isolation, PoultryAbstrak
This study aims to isolate cellulolytic bacteria derived from local chickens (Gallus gallus domesticus) and evaluate their potential in degrading cellulose in test media (CMC). Isolation was performed from the gastrointestinal tract of local chickens, which is expected to contain microorganisms with cellulose-degrading capabilities. The results of this study showed that the isolated bacteria can effectively break down cellulose, indicating their potential as probiotics that can help improve digestive health in poultry. Cellulolytic activity was tested by measuring the clear zone formed on agar media containing cellulose. The U5 bacterial isolate exhibited the highest ability to degrade cellulose substrate on agar media, marked by a clear zone of 35 mm, followed by U3 with a zone size of 33 mm. The formation of a clear zone on the CMC test medium indicates the presence of probiotic bacterial candidates, which need to be further tested for their biochemical characteristics to determine the biochemical properties of each bacterial candidate.
Referensi
Alkalbani, N. S., Turner, M. S., & Ayyash, M. M. (2019). Isolation, identification, and potential probiotic characterization of isolated lactic acid bacteria and in vitro investigation of the cytotoxicity, antioxidant, and antidiabetic activities in fermented sausage. Microbial Cell Factories, 18(1), 1–12. https://doi.org/10.1186/s12934-019-1239-1
Carrasco, J. M. D., Casanova, N. A., & Miyakawa, M. E. F. (2019). Microbiota, gut health and chicken productivity: What is the connection? Microorganisms, 7(10), 1–15. https://doi.org/10.3390/microorganisms7100374
Dar, M. A., Shaikh, A. F., Pawar, K. D., Xie, R., Sun, J., Kandasamy, S., & Pandit, R. S. (2021). Evaluation of cellulose degrading bacteria isolated from the gut-system of cotton bollworm, Helicoverpa armigera and their potential values in biomass conversion. PeerJ, 9, 1–24. https://doi.org/10.7717/peerj.11254
El-Fateh, M., Bilal, M., & Zhao, X. (2024). Effect of antibiotic growth promoters (AGPs) on feed conversion ratio (FCR) of broiler chickens: A meta-analysis. Poultry Science, 103(12), 104472. https://doi.org/10.1016/j.psj.2024.104472
Froidurot, A., & Julliand, V. (2022). Cellulolytic bacteria in the large intestine of mammals. Gut Microbes, 14(1), 1–28. https://doi.org/10.1080/19490976.2022.2031694
Fujimori, S. (2021). Humans have intestinal bacteria that degrade the plant cell walls in herbivores. World Journal of Gastroenterology, 27(45), 7784–7791. https://doi.org/10.3748/wjg.v27.i45.7784
Ghiasi, S. E., Gheibipour, M., Motamedi, H., & Dar, M. A. (2024). Screening and evaluation of the ruminal cellulolytic bacteria and their potential application as probiotics. Iranian Journal of Microbiology, 16(3), 389–400. https://doi.org/10.18502/ijm.v16i3.15796
Jannah, S. L., Moh. Anam Al Arif, Sri Chusniati, Mirni Lamid, Moh. Sukmanadi, Iwan Sahrial Hamid, & Rondius Solfaine. (2022). Potensi Pemberian Probiotik Terhadap Peningkatan Berat Badan, Konsumsi, Dan Konversi Pakan Ayam Petelur Fase Pre Layer. Media Kedokteran Hewan, 33(2), 96–104. https://doi.org/10.20473/mkh.v33i2.2022.96-104
Permadi, A., Izza, M. A., Cahyo, K., & Kholif, M. Al. (2018). Penggunaan Probiotik Dalam Budidaya Ternak. Jurnal Abadimas Adi Buana, 2(1), 5–10. https://doi.org/10.36456/abadimas.v2.i1.a1616
Raharjo, A. P., & Isnawati. (2022). Isolasi dan Karakterisasi Bakteri Selulolitik pada Pakan Fermentasi Eceng Gondok, Tongkol Jagung, dan Bekatul Padi Isolation and Characterization Cellulolytic Bacteria from Fermentation Feed of Water Hyacinth, Corncob, and Rice Bran. Lentera Bio, 11(1), 44–51. https://journal.unesa.ac.id/index.php/lenterabio/index44
Rehman, A., Arif, M., Sajjad, N., Al-Ghadi, M. Q., Alagawany, M., Abd El-Hack, M. E., Alhimaidi, A. R., Elnesr, S. S., Almutairi, B. O., Amran, R. A., Hussein, E. O. S., & Swelum, A. A. (2020). Dietary effect of probiotics and prebiotics on broiler performance, carcass, and immunity. Poultry Science, 99(12), 6946–6953. https://doi.org/10.1016/j.psj.2020.09.043
Röhe, I., & Zentek, J. (2021). Lignocellulose as an insoluble fiber source in poultry nutrition: a review. Journal of Animal Science and Biotechnology, 12(1), 1–15. https://doi.org/10.1186/s40104-021-00594-y
Sarita, B., Samadhan, D., Hassan, M. Z., & Kovaleva, E. G. (2024). A comprehensive review of probiotics and human health-current prospective and applications. Frontiers in Microbiology, 15(January), 1–14. https://doi.org/10.3389/fmicb.2024.1487641
Srifani, A., Mirnawati, Marlida, Y., Rizal, Y., & Nurmiati. (2024). Isolation and Characterization of Cellulolytic Lactic Acid Bacteria from Soymilk Waste as Probiotic Candidates for Broiler. International Journal of Veterinary Science, 13(1), 108–114. https://doi.org/10.47278/journal.ijvs/2023.067
Suryadi, M. A. F. F., Mursyid, M. H., Anwar, K., Ali, M., & Kisworo, D. (2023). Isolation of Cellulolytic Bacteria from Kalkun (Meleagris gallopavo) Gastro-Intestinal Tract as a Candidate Probiotics for Poultry. Jurnal Penelitian Pendidikan IPA, 9(5), 3981–3985. https://doi.org/10.29303/jppipa.v9i5.3739
Zubaidah, A., Prasetyo, D., Handajani, H., Rohmah, S. P., & Puspita, D. A. (2019). Screening Bakteri Selulolitik Dan Amilolitik Pada Rumen Sapi Sebagai Kandidat Probiotik Pada Budidaya Ikan Secara in Vitro. Jurnal Riset Akuakultur, 14(4), 261. https://doi.org/10.15578/jra.14.4.2019.261-271
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